FR/EN

REGULATION OF DEVELOPMENTAL NEUROGENESIS

 

 

Education and Training

  • M.Sc. in Neuroscience, University of Rouen, France, 2001
  • Ph.D. in Neuroscience, University of Rouen, France, 2005
  • Post-Doctoral Fellow, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, Piscataway, NJ USA (Emanuel DiCiccoBloom's lab), 2005-08
  • Post-Doctoral Fellow, Laboratory of Cellular and Neuroendocrine Differenciation, University of Rouen, France (David Vaudry's lab), 2008-09

Holder of a Level I training in animal experimentation and trained on small mammal surgery.

Contact Information

Inserm U1239 – DC2N
CURIB Building
2nd floor, room 239
25, rue Tesnière
76821 Mont-Saint-Aignan
Telephone: +33(0)235 14 6641
Fax: +33(0)235 14 6946
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

 

 

Educational role:

 

-          Responsible of  Master 1 Biology & Health - Cell Imaging

-          Responsible of the 1st year in Life Technologies at ESITEch Engineering School

 

Research Areas: Mechanisms controlling neuronal fate and survival, brain wiring and function

 

Research

 

Many developmental disorders arise from abnormalities in neurotransmission, neuronal connectivity, and/or neuron/glia interactions. It is thus essential to elucidate the mechanisms underlying the establishment of neuronal networks, including cortical network, to propose new therapies or markers aiming at preventing the occurrence of such brain deficits in childs. Our group is focused on the function of Selenoprotein T during neurodevelopment, and its contribution to the regulation of redox homeostasis during the establishment of functional circuitry.

 

Our research unit has characterized Selenoprotein T as a Selenium-containing protein expressed in the central nervous system during development. Using genetic invalidation models in mice, we have demonstrated that this protein contributes to the control of redox homeostasis and neuroblast survival. These alterations detected in the knockout animals lead to behavioral deficits, which manifest in the absence of gross morphological abnormalities.

 

Murine models, supplemented with more specific approaches, such as in utero electroporation are being used to contribute deciphering the mechanisms underlying the complex origin of cognitive functions, and how they can be altered during pathophysiological conditions, such as ASD or ADHD.

 

        

 

Selected Publications

DiCicco-Bloom, E., Falluel-Morel,A., Developmental Neurogenesis, in : Reference Module in Neuroscience and Biobehavioral Psychology. Elsevier, ISBN 9780128093245 in press.

Castex, M.T., Arabo, A., Bénard, M., Roy, V., Le Joncour, V., Prévost, G., Bonnet, J.-J., Anouar, Y., Falluel-Morel, A., 2015. Selenoprotein T Deficiency Leads to Neurodevelopmental Abnormalities and Hyperactive Behavior in Mice. Mol. Neurobiol. 53:5818-5832

Boukhzar, L., Hamieh, A., Cartier, D., Tanguy, Y., Alsharif, I., Castex, M., Arabo, A., El Hajji, S., Bonnet, J.-J., Errami, M., Falluel-Morel, A., Chagraoui, A., Lihrmann, I., Anouar, Y., 2016. Selenoprotein T Exerts an Essential Oxidoreductase Activity That Protects Dopaminergic Neurons in Mouse Models of Parkinson’s Disease. Antioxid. Redox Signal. 24, 557–574.

Balland, E., Dam, J., Langlet, F., Caron, E., Steculorum, S., Messina, A., Rasika, S., Falluel-Morel, A., Anouar, Y., Dehouck, B., Trinquet, E., Jockers, R., Bouret, S.G., Prévot, V., 2014. Hypothalamic tanycytes are an ERK-gated conduit for leptin into the brain. Cell Metab. 19, 293–301.

Petit, A., Delaune, A., Falluel-Morel, A., Goullé, J.-P., Vannier, J.-P., Dubus, I., Vasse, M., 2013. Importance of ERK activation in As2O3-induced differentiation and promyelocytic leukemia nuclear bodies formation in neuroblastoma cells. Pharmacol. Res. 77, 11–21.

Prevost, G., Arabo, A., Jian, L., Quelennec, E., Cartier, D., Hassan, S., Falluel-Morel, A., Tanguy, Y., Gargani, S., Lihrmann, I., Kerr-Conte, J., Lefebvre, H., Pattou, F., Anouar, Y., 2013. The PACAP-regulated gene selenoprotein T is abundantly expressed in mouse and human β-cells and its targeted inactivation impairs glucose tolerance. Endocrinology 154, 3796–3806.

Falluel-Morel, A., Lin, L., Sokolowski, K., McCandlish, E., Buckley, B., DiCicco-Bloom, E., 2012. N-acetyl cysteine treatment reduces mercury-induced neurotoxicity in the developing rat hippocampus. J. Neurosci. Res. 90, 743–750.

Sokolowski, K., Falluel-Morel, A., Zhou, X., DiCicco-Bloom, E., 2011. Methylmercury (MeHg) elicits mitochondrial-dependent apoptosis in developing hippocampus and acts at low exposures. Neurotoxicology 32, 535–544.

Tanguy, Y., Falluel-Morel, A., Arthaud, S., Boukhzar, L., Manecka, D.-L., Chagraoui, A., Prevost, G., Elias, S., Dorval-Coiffec, I., Lesage, J., Vieau, D., Lihrmann, I., Jégou, B., Anouar, Y., 2011. The PACAP-regulated gene selenoprotein T is highly induced in nervous, endocrine, and metabolic tissues during ontogenetic and regenerative processes. Endocrinology 152, 4322–4335.

DiCicco-Bloom, E., Falluel-Morel, A., 2010. Developmental Neurogenesis, in: Encyclopedia of Behavioral Neuroscience. Elsevier, pp. 396–404.

 

Funding

            

 

Current Group Members

 

  •   Anthony Falluel-Morel, Assistant Professor
  •   Emmanuelle Carpentier, Master Student

 

 

Alumni

 

  •   Lisa Brunet, Master Student, now Ph.D. student at Rouen University
  •  Valentin Vanhee, Master Student, now at CiToxLAB (Evreux, France)
  •   Francesca Trentin,  Master Student, now at Parini Hospital (Aosta, Italy)
  •   Benoît Dechelotte, Master Student, now intern in medicine (Lyon, France)
  •   Melody Atkins, Engineer student, now at UPMC (Paris, France)
  •   Matthieu Castex, Ph.D. Student, now at Lycée G. Brassens (Neufchâtel-en-Braye, France)
  •   Audrey Filézac de l’Etang, Master Student, now at Genentech (San Francisco, USA)
  •   Yannick Tanguy, Ph.D. Student, now at IPSEN Foundation (Paris, France)

 

 

 

 

Christophe DUBESSY, Associate Professor

Contact information

Inserm U1239 - DC2N (Laboratory of Neuronal and Neuroendocrine Communication and Differentiation)

CURIB building, 2nd floor, room 242

Place Emile Blondel

76821 Mont-Saint-Aignan

Tel: +33(0)235 14 6752

E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Education and Training

·         M.Sc. in Biochemistry, Molecular and Cell Biology, University of Bourgogne / Franche-Comté

·         Ph.D. in Biological Engineering - Oncology, University of Nancy I – H. Poincaré

·         Temporary research and teaching assistants (ATER, Attaché Temporaire d’Enseignement et de Recherche)

·         Post-doctoral fellow, Inserm U413 (H. Vaudry’s lab), University of Rouen

·         HDR (Habilitation à Diriger les Recherches)

·         Holder of a Level I training in animal experimentation

 

Educational Role

Co-responsible for the Licence 1 SVT degree (2006-2012)

Co-responsible for the Licence 3 SV – B2MCP degree (2012-2018)

 

Research area

Involvement of microRNAs and their targets in secretion pathologies: application to the study of secretion dysfunctions in the pheochromocytoma, a tumor of the adrenal medulla gland.

 

Current research

Regulated secretion releases neurohormones that are stored in dense core secretion vesicles (DCSV). These organelles are produced by budding from the membrane of the trans-Golgi network and transported to the plasma membrane where they will fuse in a controlled manner to release their contents into the extracellular space. Many metabolic, neuropsychiatric and neurodegenerative pathologies are associated with a deregulation of this exocytosis and the molecular mechanisms are poorly understood. We hypothesized that microRNAs (miRNAs) would be key actors. These small non-coding RNAs are regulators of gene expression that maintain homeostasis of signaling pathways; their deregulation therefore often leads to alterations in cellular and physiological functions. However, to date, very few miRNAs have been associated with exocytosis processes and without taking into account the totality of this biological process.

Our objective is therefore to study, using bioinformatics and functional approaches, the control of exocytosis by miRNAs in pheochromocytoma (PCC), a rare neuroendocrine tumor of the medullo-adrenal gland. PCC is characterized by hypersecretion of neurohormones such as catecholamines (dopamine, (nor)adrenaline), which causes harmful side effects in patients such as hypertension, which is often fatal to them.

Our studies have revealed that seven miRNAs are differentially expressed in a particular form of pheochromocytomas that secrete physiological levels of catecholamines (collaboration with A. Tabarin (CHU Bordeaux) and C. Bérard (LITIS, Rouen)). These miRNAs are also a molecular signature of these tumours.